Makler counting chamber for motility and concentration analysis

Makler counting chamber for sperm analysis

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The Makler counting chamber is a simple-to-use device for rapid and accurate sperm count, motility and morphology evaluation, from undiluted specimen.

The chamber is composed of two parts:

1. The lower part has a metal base and two handles. In the center of the base there is a flat disc made of optical flat glass on which the sample is placed.

2. The upper part is the cover glass encircled with a metal ring. At the center of its lower surface there is a 1mm2 grid, subdivided into 100 squares, each one of 0.1 x 0.1 mm.

When the cover glass is placed on the four tips, the space bounded in a row of 10 squares is exactly one millionth of mL. Therefore, the number of sperm heads in 10 squares indicates their concentration in million/mL.

More information:

Unique features and advantages:

Easy to use:
Sperm count performed from undiluted specimen.

Fast results:
The number of spermatozoa counted in any strip of 10 squares of the grid indicates their concentration in millions/mL. No additional factors are necessary for calculation.

Optimal depth:
The depth of 10 microns eliminates blurring and allows sperm to move freely. The applied sample is observed in one focal plane.

Built-in grid:
The grid is on the cover glass. This eliminates the need to insert a grid into the microscope eye piece and remove it when not required.

Economical:
Reusable. Easily cleaned with a non-bleach disinfectant solution.

Self-controlled accuracy:
Observation of color fringes at the four contact points, provides a self-controlled test for accuracy. The cover glass can never be raised by the applied sample.

Calibration unnecessary:
Repeated use with complete accuracy without calibration.

Superior technology:
Manufactured by state-of-the-art precision engineering. Checked individually by laser beam for precision and accuracy.

Accessories:

Cleaning brush.

Lint free lens paper.

Chamber grip – this device should be placed on the stage of the microscope during sperm analysis. It grips the chamber tightly and enables smooth shifting.

Additional information:

Reference: MKLR
Catalog: Download catalog pdf_thumbnail
Protocol: Download protocol pdf_thumbnail