Technical features of the SCA® DNA Fragmentation module:
Features
Manual or semi-automatic capture of the cells, (the system captures an image every few seconds), or automatic with the motorized stage.
High resolution images (768x576)
Visualization of the real image and analysis mask
Automatic recognition of the sperm with and without halo
Analysis time is 1 second per field at 20x magnification
Brightfield or fluorescence analysis is possible, depending on the kit used
custom reports (design as well as language) with images, graphics and numerical data
In the program set up the user can choose:
- the analysis according to total area or to the relation between halo and core, selecting the more convenient according to the staining.
- The way of capture: manual o automatic (with motorized stage)
Parametres offerec by the system
Total area
Halo area
Core area
Ratio
Classfication: Automatic Classification of the human sperm into fragmented and non fragmented following user´s defined criteria. (The program contains, as default, the criteria recommend by the Halosperm manufacturer, tested in the Department of Genetics of the Autonomus University of Madrid)