Kit for sperm DNA Fragmentation test

GoldCyto DNA sample

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The GoldCyto DNA kit is a simple test that allows assessment of sperm DNA Fragmentation in human.

The method is based on the Sperm Chromatin Dispersion (SCD) test (Fernández et al., J. Androl 24:59–66, 2003; Fertil Steril 84:833-842, 2005).

Intact unfixed spermatozoa are immersed in an inert agarose microgel on a pretreated slide. An initial acid treatment denatures DNA in those sperm cells with fragmented DNA. Following this, the lysing solution removes most of the nuclear proteins, and in the absence of massive DNA breakage produces nucleoids with large halos of spreading DNA loops, emerging from a central core. However, the nucleoids from spermatozoa with fragmented DNA either do not show a dispersion halo or the halo is minimal.

More information:

Information request

Kit contents:

Pretreated slides

Eppendorf tubes with low-melting-point agarose

Bottle of Phosphate Buffer Saline

Bottle of Denaturant Agent
Bottle of lysis solution

Bottle of stain solution – TA

Bottle of stain solution – TB

GoldCyto DNA kit

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Additional information:

Kit for 20 analysis:

Protocol: Download protocol pdf_thumbnail

Kit for 10 analysis:

Reference: GOLDCYTO-DNA-10
Protocol: Download protocol pdf_thumbnail