SCA Motility and concentration

SCA® Motility and concentration is a module for the automatic analysis of the sperm motility and concentration in a human semen sample.

This analysis module is also
available for Veterinary and Toxicology.

Progressive sperm motility is extensively known to be related to pregnancy rates.

Motility must be assessed as soon as possible after liquefaction of the sample, preferably at 30 minutes, but in any case within one hour after ejaculation, as WHO recommends, to decrease the effects of  dehydratation, Ph or changes in temperature.

More information:

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SCA® Motility and concentration last news

Sperm Functional Tests

Sperm Functional Tests

Automatic results of Hyperactivation and Mucus penetration.

Criteria selection

Criteria selection

Spermatozoa paths can be visualized by several criteria (WHO5, WHO4…).

Histogram of velocity

Histogram of velocity

New histogram with sperm velocity (VCL – Curvilinear Velocity).

Drift parameter

Drift parameter

Option that enables to eliminate the drift movement in the sample.

SCA® Motility and concentration main features

Automatic analysis

SCA® Motility and concentration detects the spermatozoa automatically.

Phase contrast analysis

Samples are analysed under phase contrast (positive phase contrast).

Count and motility simultaneously

Accurate count and motility analysis are carried simultaneously.

Kinematic parameters

Motile sperm is analysed by following the sperm trajectory and the velocity. The results, comprising all kinematic parameters for CASA, are then presented within seconds.

Results

SCA® is a powerful tool for Andrology, as CASA is the only objective method to analyse the total progressive, motile and hyperactive spermatozoa in a sample, hence giving the indication of the sperm fertilizing potential.

Intelligent filter

The ultimate SCA® version incorporates the innovation of an intelligent filter, is capable to detect the tail of the spermatozoa, thus permitting the correct analysis of samples containing debris.

Additional information

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Creating new analysis configurations icon_correct icon_correct
Modification of the fps images capture velocity icon_correct icon_correct
Modification of the optics, microscope scales and image capture options icon_correct icon_correct icon_correct
Choosing analysis chamber (Leja®, Makler®, etc) icon_correct icon_correct icon_correct
Motorised stage Prior optiscan III icon_correct icon_correct
Particle area selection (sperm head area) icon_correct icon_correct icon_correct
VCL  or VAP, STR, LIN, VAP, Connectivity, Sort, Analysis restrictions icon_correct icon_correct
Species: Human icon_correct icon_correct icon_correct
Species: All (human, and all animals, including rat and mouse) icon_correct
Species: All animals with oval sperm head icon_correct
Species: rat and mouse icon_correct
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Specific digital Basler camera with 780×580 resolution and 25 fps capture velocity icon_correct icon_correct icon_correct
Specific digital Basler camera with 1280×1024 resolution and 25 fps capture velocity icon_correct icon_correct icon_correct
Specific digital Basler camera with 780×580 resolution and up to 75 fps capture velocity icon_correct icon_correct
Specific digital Basler camera with 1280×1024 resolution and up to 200 fps capture velocity icon_correct icon_correct
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Total sperm counted icon_correct icon_correct icon_correct
WHO 5: Total and percentage of the counted sperm that are Static, Non progressive and progressive icon_correct icon_correct icon_correct
Concentration in M/ml and in the sample volume icon_correct icon_correct icon_correct
Concentration in M/ml and in the sample volume of Static, Non-progressive and Progressive icon_correct icon_correct
Total analysed and Percentage of the Rapid, Medium, Slow and Static icon_correct icon_correct icon_correct
Concentration in M/ml and in the sample volume  the Rapid, Medium, Slow and Static icon_correct icon_correct
WHO 4: Total analysed and percentage of Type A, B, C and D icon_correct icon_correct icon_correct
WHO 4: Concentration in M/ml and in the sample volume of the Type A, B, C and D. icon_correct icon_correct
Head area average in square µm icon_correct icon_correct icon_correct
Head area in Static, Slow, Medium and Rapid spermatozoa analysed icon_correct icon_correct
Average of Round Cells in M/ml icon_correct icon_correct icon_correct
Total Round Cells concentration in M/ml icon_correct icon_correct
Total analysed and percentage of Circular tracks icon_correct icon_correct
Average of VCL, VSL, VAP, LIN, STR, WOB in analysed spermatozoa icon_correct icon_correct icon_correct
Average of VCL, VSL, VAP, LIN, STR, WOB  on Slow, Medium and Rapid  Spermatozoa analysed icon_correct icon_correct
Total and percentage of Hyperactive spermatozoa analysed icon_correct icon_correct icon_correct
Concentration of Hyperactive spermatozoa M/ml and M/Sample icon_correct icon_correct
Average values of ALH, and BCF in analysed spermatozoa icon_correct icon_correct icon_correct
ALH and BCF values in Slow and Rapid progressive sperm icon_correct icon_correct
Captured images and distribution charts icon_correct icon_correct
Captured Videos icon_correct icon_correct
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SCA® dataShare icon_correct icon_correct
SCA® Stage Controller and Prior Motorized Stage icon_correct icon_correct
SCA® Capture icon_correct icon_correct
SCA® Viewer icon_correct icon_correct
SCA® Editor icon_correct icon_correct
SCA® Droplets icon_correct
Motility and concentration are analyzed in Phase contrast microscopy (an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image These phase shifts, although invisible, become visible when shown as brightness variations allowing the clear visualization of the semen sample).
Or Fluorescence (The specimen is illuminated with light of a specific wavelength which is absorbed by the fluorochromes, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light)
Images are captured with high frame rate digital camera Basler (25 , 50, 75 or up to 200 fps)
Automatic detection of non homogeneous fields, allowing a consistent analysis
There is a sort function that permits to create groups of sperm population, useful in any sperm research.
Modifiable parameters: Particle area for sperm detection, Velocity Classification, VAP, Frame rate, Connectivity, Circularity, Parameter restriction, for example as elongation (to improve the cell detection)
Other modifiable parameters: color mask of the path.
Allows capture up to 30 fields of the sample
Capture time is 1 second per field
Intelligent filter that automatically eliminate captured debris in human semen samples
Compatible with several disposable analysis chambers like Leja® or Makler® counting chamber
Counting
Kinematic parameters:

VCL, curvilinear velocity (m/s). Time-averaged velocity of a sperm head along its actual curvilinear path, as perceived in two dimensions in the microscope. A measure of cell vigour.

VSL, straight-line (rectilinear) velocity (µm/s). Time-averaged velocity of a sperm head along the straight line between its first detected position and its last.

VAP, average path velocity (µm/s). Time-averaged velocity of a sperm head along its average path. This path is computed by smoothing the curvilinear trajectory according to algorithms in the SCA Motility module.

ALH, amplitude of lateral head displacement (µm). Magnitude of lateral displacement of a sperm head about its average path. It can be expressed as a maximum or an average of such displacements.

LIN, linearity. The linearity of a curvilinear path, VSL/VCL.

WOB, wobble. A measure of oscillation of the actual path about the average path, VAP/VCL.

STR, straightness. Linearity of the average path, VSL/VAP.

BCF, beat-cross frequency (Hz). The average rate at which the curvilinear path crosses the average path.
Sample (ejaculate or straws) Concentration in M/ml.
Total analysed spermatozoa , Percentage in the sample, concentration in M/ml and in the sample volume of the spermatozoa classified by:
Velocity: Rapid, Medium, slow and Static
WHO 4 classification: Type A (rapid and progressive), B (medium progressive), C(non progressive) and D (static or immotile)
WHO 5 classification: Progressive (Type A+B), Motile (Type A+B+C), Static (Type D)
Hyperactive
Average of the kinematic parameters presented in the sample
Total of the head Area / velocity type
Total of Round cells / velocity type
Total and percentage of circular tracks
ALH and BCF in Medium and Rapid Progressive Sperm

Find further explanations of the CASA Terminology in WHO Laboratory Manual for the Examination and processing of human semen.

Download the WHO manual pdf_thumbnail

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