SCA® Morphology is a module that permits the automatic morphometry analysis and morphology classification of human stained sperm samples observed in brightfield microscopy.
| This analysis module is also available for Veterinary and Toxicology. |
There are enormous variation in the sperm morphology, which make its assessment quite complex, but several studies have recovered spermatozoa from female reproductive tract, specially in postcoital endoncervical mucus, and from the surface of zona pellucida and have helped to define the appearance of potentially fertilizing, that is, morphologically normal, spermazoa in some species. (Medkveld et al,. 1991: Liu & Baker, 1992a)
More information:
SCA® Morphology last news
SCA® Morphology last news
Vacuoles and tails
Automatic analysis of vacuoles and tails with SCA® Morphology.
Morphology slides SCA
Ready for the new Morphology slides SCA pre-stained slides.
Multiple defects
Assessment of all possible defects at the same time: TZI, MAI and SDI.
Analysis improved
Morphology analysis and sperm detection improved.
SCA® Morphology main features
SCA® Morphology main features
SCA® Morphology detects and analyses the spermatozoa automatically.
Simultaneously evaluates the dimensions of the acrosome, head, midpiece, tail and the presence of vacuoles.
Spermatozoa are recorded as normal or abnormal, following established criteria: W.H.O., Strict Kruger-Tygerberg, David modified, Adaptative or Customized.
Optionally, analysis with motorized stage and SCA® Stage controller is available, improving the capture velocity and making all the process automatic.
Several staining are compatible: Sperm Stain, SpermBlue, Diff-Quik, Papanicoulau, Shorr or Morphology slides SCA and SCA® contains the appropriate correction factors. *
SCA® Morphology is an effective and user-friendly tool for research, allowing the implementation of new terminology and estimations in sperm morphology.
* Morphometric dimensions of the human sperm head depend on the staining method used
L. Maree, S.S. du Plessis, R. Menkveld and G. van der Horst
Additional information
Additional information
| Human Edition |
Research Edition |
|
| Creating new analysis configurations |  |
|
| Modification of analysis box size | |
|
| B/W or color capture, Scale, image capture options | |
|
| Motorized stage | |
|
| Human classification: W.H.O. or David Modified Classification |
|
|
| Human classification: W.H.O, Kruger-tygerberg, David modified and User defined | |
|
| All staining options: Diff-Quik, Sperm Stain, SpermBlue®, Hemacolor, Papanicolau, Shorr, Cell-Vu Prestained Slides. | |
|
| Particle area modification | |
|
| Modification of the Morphology classification / Morphometric values | |
|
| Species available: Human | |
|
| Species available: All (human, and all animals, including rat and mouse) | – | |
| Species available: All animals with oval sperm head (boar, bull, stallion, etc) | – | |
| Species available: rat and mouse | – | |
| Human Edition |
Research Edition |
|
| Specific digital Basler camera with 780×580 resolution | |
|
| Specific digital Basler camera with 1280×1024 resolution | |
|
| Human Edition |
Research Edition |
|
| Total and percentage normal/abnormal sperm analysed | |
|
| Basic classification: normal and head, midpiece and tail defects, cytoplasmic droplets | |
|
| TZI, MIA, SDI, ERC Index | |
|
| Total analyzed and percentage of the spermatozoa with the next morphologic values (*): normal size, acrosome and midpiece; micro, macro, normal shape, tapered, thin, round, pyriform, amorphous, abnormal midpiece in size, angle, insertion, normal and abnormal tail, short, irregular, rolled, multiple, without, angulation. * Morphologic values might change depending on the classification selected. |
|
|
| Morphometric values: head length, width,area, perimeter, ellipticity, elongation, surface roughness, regularity | |
|
| Captured images and distribution charts | |
|
| Human Edition |
Research Edition |
|
| SCA® dataShare | |
|
| SCA® Stage Controller |
|
|
| SCA® Capture | |
|
| SCA® Viewer | |
|
| SCA® Editor | |
|
| Analysis under brightfield microscopy, transmitting light to view a specimen that is stained. |
| SCA Morphology analyzes the head, acrosome and midpiece form, shape and size. |
| Tails can be analyzed automatically with the use of the specific high resolution digital camera 1280×1024 or manually if desired. |
| Automatic vacuoles analysis is available using the Sperm Blue® staining kit. |
| There are several Compatible staining methods depending on the specimen: Diff quik, Papanicoulau, Sperm Blue®, Sperm Stain, Shorr and CellVu prestained slides for human. Sperm Blue® for all species, which allows a complete analysis, including acrosome visualization. For each of the staining used the software has corrected factors. |
| Allows the creation of User defined matrix for the classification into normal or abnormal sperm |
| Additional descriptions of the Morphologic values can be created |
| Modifiable parameters are: Size of the capture box, staining methods and Classification criteria |
| SCA® permits to capture and analyse up to 500 spermatozoa per sample |
| Head length, width, area, perimeter, ellipticity, elongation, roughness, regularity |
| Acrosome, percentage of head size |
| Vacuoles, presence and percentage of head size (with SpermBlue® staining) |
| Midpiece width, area, insertion angle |
| Tail length |
| Calculation of the following basic parameters: Total analyzed and Percentage of normal and abnormal spermatozoa |
| Total analyzed and Percentage of normal spermatozoa; with head, midpiece and tail defects; and cytoplasmic droplets. |
| Total and percentage of counted spermatozoa having Just one defect, in the Head, Midpiece or Tail. |
| Total and percentage of counted spermatozoa having Multiple defects combined as: Head+ Midpiece, Head+Tail, Midpiece+Tail, Head+Midpiece+Tail |
| Morphologic values*: Total and percentage of analysed spermatozoa having Normal size, Micro, Macro, Normal shape, Tapered, Thin, Round, Pyriform, Amorphous; Normal and Abnormal Acrosome; Normal midpiece, Abnormal midpiece size, angle and insertion; Normal tail, abnormal, short, Irregular, Rolled, Multiple, Without, Angulation |
| Average and deviation of these morphometric values: Head lengh, width, area, perimeter, ellipticity, elongation, roughness, regularity; Midpiece width, area and angle; Acrosome |
| Teratozoospermy index (TZI), Deformity index(SDI), Multiple anomalies index (MIA), Number of spermatozoa with Excess residual cytoplasm (ERC) |
* this might change according the chosen Morphology classification
Image gallery
Image gallery
Video
Video
Links
Links
| View SCA® Human catalog online |
Download SCA® Human catalog  |
| Other documents and support: Articles, Manuals, Tutorials, Protocols, mySCA, Technical request |