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DNA
Kit
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SPERM-HALOMAX®:
THE EASY WAY TO MEASURE DNA FRAGMENTATION IN SPERM SAMPLES
The SPERM-HALOMAX® kit series from CHROMACELL SL
bursts into the market as a pioneering product for the
analysis of DNA fragmentation levels in spermatozoa
both in domestic and experimental animals. SPERM-HALOMAX®
is a simple test to study DNA fragmentation levels in
spermatozoa. The method is a variant of the Sperm Chromatin
Dispersion protocol currently employed in humans (SCD;
Fernández et al., 2003. J. Androl. 24: 49-66).
This new methodology provides a simple way to distinguish,
even at low magnification, spermatozoa with and without
fragmented DNA, giving high quality images both under
fluorescence and light microscopy.
Species available: boar, bull, mouse, ram, goat, stallion
and fish.
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Figure. Sperm sample analyzed with SPERM-HALOMAX®.
Images were obtained using a 40x objective under bright
field microscopy (above) and using fluorescence microscopy
after DNA In Situ Nick Translation (below).
Large halos of chromatin dispersion in both images
correspond to sperm cells with fragmented DNA.
Authorized distributor of ChromaCell
SL products
SCA-DNAF
also availabe
SCA-DNAF:
Specific software to analyze sperm DNA fragmentation.
The software evaluates the sample automatically and
produces detailed numerical results of DFI (DNA Fragmentation
Index) according to the size of the halos.
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