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Makler
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The
Makler Chamber for SCA
Chamber Description: The Makler Chamber for
SCA is only 10 microns deep, making it the shallowest
of known chambers. Constructed from two pieces of optically
flat glass, the upper layer serves as a cover glass.
Spacing is firmly secured by four quartz pins.
A cover glass with grid is also available (a 1 sq.mm
fine grid in the center subdivided into 100 squares
of 0.1 x 0.1 mm each).
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Advantages:
- Applied spermatozoa are uniformly distributed
and monolayered, and are observed in one focal plane.
- Dilution is unnecessary even with concentrated specimens.
Analysis is done directly from original specimen in
its natural environment.
- All spermatozoa acquire friction free, horizontal
movement and are always examined under constant conditions.
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- The fact
that sperm motility is examined each time under identical
conditions further increases accuracy. Errors incurred
by uncontrolled pressure applied to the cover slip are
thus avoided.
- The 10 micron depth of the Makler Chamber is ideal
for still or movie camera photomicrography, as it approximately
matches the field depth of the objective used in semen
analysis.
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