V\u00edctor J Atencio Garc\u00eda, MSc; Jos\u00e9 A Espinosa, MSc; Jos\u00e9 G Mart\u00ednez, MSc; Sandra C Pardo Carrasco, PhD<\/p>\n
Centro de Investigaci\u00f3n Pisc\u00edcola, Facultad de Medicina Veterinaria y Zootecnia, Universidad de C\u00f3rdoba; Laboratory of Animal Genetics and Evolution \u2013 LEGAL, Institute of Biological Sciences, Federal University of Amazonas, Manaus, Brasil; Facultad de Ciencias Agrarias, Departamento de Producci\u00f3n Animal, BIOGEM, Universidad Nacional de Colombia Sede Medell\u00edn, Colombia<\/em><\/p>\n Background: cryopreservation is an important biotechnological tool in the conservation of biodiversity, particularly for endangered species. Objective: to evaluate six different spermatozoa\/oocyte ratios using fresh and cryopreserved semen in bocachico fish (Prochilodus magdalenae). Methods: fresh semen was collected and its quality determined to verify cryopreservation feasibility. The semen was put in 5 mL straws and mixed with a solution (5.5% glucose, 12% egg yolk, and 10% dimethyl sulfoxide \u2013DMSO-) in a 1:4 dilution (semen:solution). The semen was frozen in nitrogen vapor dry shipper for 30 min, rapidly transferred to storage thermos, and submerged directly into liquid nitrogen (LN; -196 \u00b0C). Straws were thawed at 60 \u00baC for 45 seconds. Motility, velocity, and sperm progressivity of fresh and cryopreserved semen were assessed using Sperm Class Analyzer (SCA\u00ae) software. Each proportion of spermatozoa\/oocyte was assessed with 2 g of eggs (1,630 \u00b1 87 eggs\/g) to evaluate fertility (F), hatching (H), and larval survival (LS) rates. Results: the best reproductive performance for fresh semen was obtained inseminating with 160,000 spermatozoa\/oocyte (F = 75.0%, H = 67.7%, LS = 32.7%). Similarly, the best reproductive performance for cryopreserved semen was achieved with 320,000 spermatozoa\/oocyte (F = 70.0%, H = 48.6%, LS = 19.5%). Conclusion: it is possible to achieve adequate reproductive performance in bocachico fish using cryopreserved sperm (10% DMSO, 5.5% glucose, and 12% egg yolk) at twice the spermatozoa\/oocyte ratio used with fresh semen.<\/p>\n doi: 10.17533\/udea.rccp.v28n4a07<\/p>\n","protected":false},"excerpt":{"rendered":"