{"id":32091,"date":"2020-03-04T13:17:01","date_gmt":"2020-03-04T12:17:01","guid":{"rendered":"https:\/\/www.micropticsl.com\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/"},"modified":"2020-03-30T13:25:08","modified_gmt":"2020-03-30T11:25:08","slug":"does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls","status":"publish","type":"avada_faq","link":"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/","title":{"rendered":"Does single\u2010layer centrifugation with Bovicoll improve sperm quality of frozen\u2010thawed semen in Fleckvieh bulls?"},"content":{"rendered":"<h3>Does single\u2010layer centrifugation with Bovicoll improve sperm quality of frozen\u2010thawed semen in Fleckvieh bulls?<\/h3>\n<p>Joanna Szlendak, Christine Adler, Jakob Scherzer, Anna Niwinska, Ewa Kautz, Ricardo Faundez<\/p>\n<p><em>Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences WULS \u2013<br \/>\nSGGW, Warsaw, Poland; Bayern-Genetik GmbH, Kumhausen, Germany<br \/>\n<\/em><\/p>\n<p><strong>Abstract:\u00a0<\/strong>The aim of the present study was to evaluate the effect of sperm selection by single\u2010layer centrifugation (SLC) performed before freezing on sperm quality after thawing of Fleckvieh bull semen. Ejaculates from 22 bulls were collected by artificial vagina and divided into two aliquots. One aliquot (control sample) was diluted with Steridyl\u00ae and frozen over nitrogen vapour in a Digitcool freezer (IMV Technologies). Sperm from the second aliquot (SLC sample) was selected using the SLC technique with Bovicoll colloid and then frozen over nitrogen vapour in a Digitcool freezer. After thawing, both samples (control and SLC) were evaluated by computer\u2010aided sperm analysis (CASA; SCA 6.4 System; Microptic S.L) for sperm motility parameters. Integrity of the plasma membrane (viability), high mitochondrial membrane potential (HMMP) and acrosome integrity were assessed using a Guava\u00ae easyCyte flow cytometer (IMV Technologies). Morphological examination of spermatozoa was performed by Differential Interference Contrast microscopy (Leica DMi8). Morphological examination of live, immobilized spermatozoa was analysed under high magnification (\u22656,600\u00d7). After thawing, the mean sperm viability of the control sample was 51.57%, compared to 40.37% for the SLC sample (p &lt; .01). HMMP was higher (p &lt; .01) in the control sample (40.37% versus 28.96%), and the mean of live spermatozoa with damaged acrosome was significantly higher (p &lt; .03) in the SLC sample (1.63% versus 1.95%). The mean percentage of motile spermatozoa was 80.17% in the control sample, compared to 75.14% in the SLC sample (p &lt; .0195), and rapid subpopulation reduced from 20.08% to 8.99% (p &lt; .0001) after SLC. Percentage of hyperactivated sperm decreased from 12.23% to 4.28% (p &lt; .0001) after SLC. Given the overall results, the sperm quality of thawed Fleckvieh bull semen was not improved when sperm were selected by SLC before freezing.<\/p>\n<p>Reproduction in Domestic Animals &#8211; https:\/\/doi.org\/10.1111\/rda.13666<br \/>\nReceived: 11 November 2019 \/ Accepted: 27 February 2020 \/ First published: 04 March 2020<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Does single\u2010layer centrifugation with Bovicoll improve sperm quality of frozen\u2010thawed semen in Fleckvieh bulls? Joanna Szlendak, Christine Adler, Jakob Scherzer, Anna Niwinska, Ewa Kautz, Ricardo Faundez Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences WULS \u2013 SGGW, Warsaw, Poland; Bayern-Genetik GmbH, Kumhausen, Germany Abstract:\u00a0The aim of the <a href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/\"> [&#8230;]<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"faq_category":[148,157],"class_list":["post-32091","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-animal-es","faq_category-bull-es"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Does single\u2010layer centrifugation with Bovicoll improve sperm quality of frozen\u2010thawed semen in Fleckvieh bulls? - HT CASA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single\u2010layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen\u2010thawed-semen-in-fleckvieh-bulls\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Does single\u2010layer centrifugation with Bovicoll improve sperm quality of frozen\u2010thawed semen in Fleckvieh bulls? - HT CASA\" \/>\n<meta property=\"og:description\" content=\"Does single\u2010layer centrifugation with Bovicoll improve sperm quality of frozen\u2010thawed semen in Fleckvieh bulls? Joanna Szlendak, Christine Adler, Jakob Scherzer, Anna Niwinska, Ewa Kautz, Ricardo Faundez Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences WULS \u2013 SGGW, Warsaw, Poland; Bayern-Genetik GmbH, Kumhausen, Germany Abstract:\u00a0The aim of the [...]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single\u2010layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen\u2010thawed-semen-in-fleckvieh-bulls\/\" \/>\n<meta property=\"og:site_name\" content=\"HT CASA\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/HamiltonThorneInc\" \/>\n<meta property=\"article:modified_time\" content=\"2020-03-30T11:25:08+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@HamiltonThorne\" \/>\n<meta name=\"twitter:label1\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"TechArticle\",\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/\"},\"author\":{\"name\":\"microptic\",\"@id\":\"https:\/\/www.micropticsl.com\/#\/schema\/person\/f44383b138fe27e5c0ed406429b01139\"},\"headline\":\"Does single\u2010layer centrifugation with Bovicoll improve sperm quality of frozen\u2010thawed semen in Fleckvieh bulls?\",\"datePublished\":\"2020-03-04T12:17:01+00:00\",\"dateModified\":\"2020-03-30T11:25:08+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/\"},\"wordCount\":357,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/www.micropticsl.com\/#organization\"},\"inLanguage\":\"es\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/\",\"url\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/does-single%e2%80%90layer-centrifugation-with-bovicoll-improve-sperm-quality-of-frozen%e2%80%90thawed-semen-in-fleckvieh-bulls\/\",\"name\":\"Does single\u2010layer centrifugation with Bovicoll improve sperm quality of frozen\u2010thawed semen in Fleckvieh bulls? 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