{"id":32480,"date":"2020-02-29T15:35:11","date_gmt":"2020-02-29T14:35:11","guid":{"rendered":"https:\/\/www.micropticsl.com\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/"},"modified":"2020-02-29T15:35:11","modified_gmt":"2020-02-29T14:35:11","slug":"a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers","status":"publish","type":"avada_faq","link":"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/","title":{"rendered":"A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers"},"content":{"rendered":"<h3>A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers<\/h3>\n<p>Giusy Rusco, Michele Di Iorio, Roberta Iampietro, Stefano Esposito, Pier Paolo Gibertoni, Maurizio Penserini, Alessandra Roncarati and Nicolaia Iaffaldano<\/p>\n<p><em>Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, Italy; Mediterranean Trout Research Group \u201cMTRG\u201d, 42037 Collagna RE, Italy; School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino MC, Italy<br \/>\n<\/em><\/p>\n<p><strong>Abstract: <\/strong>The aim of our study was to test the effectiveness of a simple semen cryopreservation procedure, developed for cultivated salmonid, on the wild salmonid of the Mediterranean area and to evaluate the effect of different thawing rates and sperm-to-egg ratios. The semen of five individual males was diluted into a final extender concentration of 0.15 M glucose and 7.5% methanol and loaded into 0.25 mL plastic straws, and a final sperm concentration of 3.0 \u00d7 109 sperm\/mL was obtained. After equilibration, the straws were frozen by exposure to liquid nitrogen vapor at 3 cm above the liquid nitrogen level for 5 min. The semen was thawed at 40 \u00b0C\/5 s or 10 \u00b0C\/30 s. The sperm cryosurvival was evaluated by examining in vitro the sperm motility parameters using the CASA system, followed by fertilization trials in vivo, using three different sperm-to-egg ratios 6 \u00d7 105, 4.5 \u00d7 105 and 3 \u00d7 105:1. The applied cryopreservation procedure resulted in remarkably high (85.6%) post-thaw sperm total motility, when the semen was thawed at 40 \u00b0C\/5 s, whilst the highest fertilization rate (53.1%) was recorded for a sperm-to-egg ratio of 4.5 \u00d7 105:1. According to these outcomes, the cryopreservation procedure that was tested turned out to be effective for the wild population of Mediterranean brown trout and practical for the creation of the first European semen cryobank foreseen as part of our \u201cLIFE\u201d Nat.Sal.Mo. project.<\/p>\n<p>See full article <a href=\"https:\/\/www.mdpi.com\/2076-2615\/10\/3\/403\/htm\" target=\"_blank\" rel=\"noopener noreferrer\">here<\/a><\/p>\n<p>Animals 2020, 10(3), 403; https:\/\/doi.org\/10.3390\/ani10030403<br \/>\nReceived: 3 February 2020 \/ Revised: 19 February 2020 \/ Accepted: 26 February 2020 \/ Published: 29 February 2020<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers Giusy Rusco, Michele Di Iorio, Roberta Iampietro, Stefano Esposito, Pier Paolo Gibertoni, Maurizio Penserini, Alessandra Roncarati and Nicolaia Iaffaldano Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, Italy; Mediterranean Trout <a href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/\"> [&#8230;]<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"faq_category":[148,217],"class_list":["post-32480","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-animal-es","faq_category-fish-es"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers - HT CASA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers - HT CASA\" \/>\n<meta property=\"og:description\" content=\"A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers Giusy Rusco, Michele Di Iorio, Roberta Iampietro, Stefano Esposito, Pier Paolo Gibertoni, Maurizio Penserini, Alessandra Roncarati and Nicolaia Iaffaldano Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, Italy; Mediterranean Trout [...]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/\" \/>\n<meta property=\"og:site_name\" content=\"HT CASA\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/HamiltonThorneInc\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@HamiltonThorne\" \/>\n<meta name=\"twitter:label1\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"TechArticle\",\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/\"},\"author\":{\"name\":\"microptic\",\"@id\":\"https:\/\/www.micropticsl.com\/#\/schema\/person\/f44383b138fe27e5c0ed406429b01139\"},\"headline\":\"A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers\",\"datePublished\":\"2020-02-29T14:35:11+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/\"},\"wordCount\":320,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/www.micropticsl.com\/#organization\"},\"inLanguage\":\"es\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/\",\"url\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/a-simple-and-efficient-semen-cryopreservation-method-to-increase-the-genetic-variability-of-endangered-mediterranean-brown-trout-inhabiting-molise-rivers\/\",\"name\":\"A Simple and Efficient Semen Cryopreservation Method to Increase the Genetic Variability of Endangered Mediterranean Brown Trout Inhabiting Molise Rivers - 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