{"id":32499,"date":"2020-03-13T16:46:07","date_gmt":"2020-03-13T15:46:07","guid":{"rendered":"https:\/\/www.micropticsl.com\/faq-items\/effect-of-semen-extenders-on-viability-of-isa-brown-and-hubbard-flex-roosters-sperm-stored-for-24-h\/"},"modified":"2020-03-13T16:46:07","modified_gmt":"2020-03-13T15:46:07","slug":"effect-of-semen-extenders-on-viability-of-isa-brown-and-hubbard-flex-roosters-sperm-stored-for-24-h","status":"publish","type":"avada_faq","link":"https:\/\/www.micropticsl.com\/es\/faq-items\/effect-of-semen-extenders-on-viability-of-isa-brown-and-hubbard-flex-roosters-sperm-stored-for-24-h\/","title":{"rendered":"Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters&#8217; sperm stored for 24 h"},"content":{"rendered":"<h3>Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters&#8217; sperm stored for 24\u00a0h<\/h3>\n<p>Ewa \u0141ukaszewicz, Anna Jerysz and Artur Kowalczyk<\/p>\n<p><em>Division of Poultry Breeding, Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Wroc\u0142aw, Poland<\/em><\/p>\n<p><strong>Abstract: <\/strong>Artificial insemination is used in almost 95% of turkey reproductive flocks and is becoming more important in chickens, particularly broiler breeders, as well as in assisted reproduction of wild birds kept in breeding centers. Diluted semen is recommended for artificial insemination. Pooled semen samples collected twice a week by dorso-abdominal massage from 2 chicken lines: laying\u2014ISA Brown (ISA-B) and meat type\u2014Hubbard Flex (H-F) were divided into 5 parts: neat semen and diluted in 1:2 ratio with 4 extenders: basic EK; EK + 1\u00a0\u03bcg\/mL organic selenium and 8\u00a0\u03bcg\/mL vitamin E; EK + 10\u00a0mg\/mL of royal jelly; and EK + 0.25\u00a0g\/mL of lyophilized bovine colostrum. Diluted semen samples were evaluated after 15\u00a0min and then 24\u00a0h storage at 4\u00b0C. Sperm concentration, motility, motility parameters (with Sperm Class Analyzer), and morphology were evaluated in the neat semen, whereas in diluted and stored samples, the last 3 traits were determined. In case of both lines, dilution did not affect (P &gt; 0.05) the number of live normal cells (78.0\u201381.1% in ISA Brown and 73.8\u201368.7% in Hubbard Flex) in relation to neat semen; however, bovine colostrum addition increased (P &lt; 0.05) the percentage of bulb head sperm (5.7 vs. 10.0% and 12.1 vs. 17.6%, for ISA and Hubbard, respectively) and decreased sperm motility (67.4 vs. 92.9% and 67.3 vs. 98.5% for ISA and Hubbard). The 24\u00a0h storage of neat semen and semen diluted with colostrum caused (P &lt; 0.05) the unfavorable changes in all evaluated traits and both chicken lines, whereas semen dilution with remaining extenders decreased the percentage of live normal cells (by 18.8\u201323.4% ISA and by 20.9\u201325.5% Hubbard) but did not affect sperm motility (81.5\u201387.6% for ISA and 81.1\u201396.6% for Hubbard). Sperm motility and motility parameters depended both on the extender and chicken line.<\/p>\n<p>See full article <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0032579120300730\" target=\"_blank\" rel=\"noopener noreferrer\">here<\/a><\/p>\n<p>Poultry Science &#8211; https:\/\/doi.org\/10.1016\/j.psj.2019.12.055<br \/>\nRevised 11 December 2019, Accepted 12 December 2019, Available online 13 March 2020<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters&#8217; sperm stored for 24\u00a0h Ewa \u0141ukaszewicz, Anna Jerysz and Artur Kowalczyk Division of Poultry Breeding, Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Wroc\u0142aw, Poland Abstract: Artificial insemination is used in almost 95% of turkey reproductive flocks and is <a href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/effect-of-semen-extenders-on-viability-of-isa-brown-and-hubbard-flex-roosters-sperm-stored-for-24-h\/\"> [&#8230;]<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"faq_category":[148,244],"class_list":["post-32499","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-animal-es","faq_category-bird-es"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters&#039; sperm stored for 24 h - HT CASA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/effect-of-semen-extenders-on-viability-of-isa-brown-and-hubbard-flex-roosters-sperm-stored-for-24-h\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters&#039; sperm stored for 24 h - HT CASA\" \/>\n<meta property=\"og:description\" content=\"Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters&#8217; sperm stored for 24\u00a0h Ewa \u0141ukaszewicz, Anna Jerysz and Artur Kowalczyk Division of Poultry Breeding, Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Wroc\u0142aw, Poland Abstract: Artificial insemination is used in almost 95% of turkey reproductive flocks and is [...]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.micropticsl.com\/es\/faq-items\/effect-of-semen-extenders-on-viability-of-isa-brown-and-hubbard-flex-roosters-sperm-stored-for-24-h\/\" \/>\n<meta property=\"og:site_name\" content=\"HT CASA\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/HamiltonThorneInc\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@HamiltonThorne\" \/>\n<meta name=\"twitter:label1\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"TechArticle\",\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/effect-of-semen-extenders-on-viability-of-isa-brown-and-hubbard-flex-roosters-sperm-stored-for-24-h\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/effect-of-semen-extenders-on-viability-of-isa-brown-and-hubbard-flex-roosters-sperm-stored-for-24-h\/\"},\"author\":{\"name\":\"microptic\",\"@id\":\"https:\/\/www.micropticsl.com\/#\/schema\/person\/f44383b138fe27e5c0ed406429b01139\"},\"headline\":\"Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters&#8217; 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