{"id":34671,"date":"2022-03-22T15:48:25","date_gmt":"2022-03-22T14:48:25","guid":{"rendered":"https:\/\/www.micropticsl.com\/faq-items\/assessment-of-sperm-viability-and-computer-assisted-motility-analysis-in-budgerigars-melopsittacus-undulatus-effect-of-several-in-vitro-processing-conditions\/"},"modified":"2022-03-22T15:48:25","modified_gmt":"2022-03-22T14:48:25","slug":"assessment-of-sperm-viability-and-computer-assisted-motility-analysis-in-budgerigars-melopsittacus-undulatus-effect-of-several-in-vitro-processing-conditions","status":"publish","type":"avada_faq","link":"https:\/\/www.micropticsl.com\/es\/faq-items\/assessment-of-sperm-viability-and-computer-assisted-motility-analysis-in-budgerigars-melopsittacus-undulatus-effect-of-several-in-vitro-processing-conditions\/","title":{"rendered":"Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions"},"content":{"rendered":"

Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions<\/h3>\n

Manuela Madeddu, Stefano Marelli, Ahmad Abdel Sayed, Fabio Mosca, Silvia Cerolini and Luisa Zaniboni<\/p>\n

Department of Veterinary Medicine and Animal Sciences, University of Milan, via dell\u2019Universit\u00e0 6, Lodi 26900, Italy<\/em><\/p>\n

Abstract:<\/strong> In order to preserve endangered psittacine species, more basic and applied research in reproductive biology is required. Assisted reproductive technologies such as artificial insemination play an important role in parrots species conservation programs to overcome the problem of infertile eggs and male infertility. The aim of this study was to define an effective in vitro protocol in order to standardize the sperm quality evaluation in psittacines, studying Melopsittacus undulatus as model species. Semen was collected from twenty adult males by massage technique from May to June. Sperm concentration was measured by the spectrophotometric method. Sperm quality (sperm membrane integrity (SMI), motility, and kinetic parameters) was assessed on fresh semen. Three different experimental protocols were performed to compare the effects of various processing conditions on SMI, motility, and kinetic parameters. In protocol 1, test was performed by Lake extender with three different pH, 7.4 versus 8.2 versus 8.4, and two different equilibration temperatures after dilution of fresh semen (4\u00b0C versus 25\u00b0C). In protocol 2, two dilution rates of semen after collection were valuated, 1\u2009:\u20093 versus 1\u2009:\u20094, as well as three different semen storage temperatures (4\u00b0C versus 25\u00b0C versus 38\u00b0C) before sperm motility analysis with the computer-assisted sperm analysis (CASA). In protocol 3, two different Makler chamber temperatures (38 versus 41\u00b0C) during motility analysis were tested. A significant progressive improvement in spermatozoa motility and kinetic parameters was registered with pH 8.4. Progressive motility and all kinetic parameters were higher at 4\u00b0C equilibration temperature. Straightness (STR) kinetic parameter was better with 1\u2009:\u20094 dilution rate. Total motile sperm was higher in 41\u00b0C Makler chamber. In this study, for the first time, the effects of different processing protocols on psittacines seminal quality analysis were investigated. Significant differences conditioning the effectiveness of analysis protocols have been described.<\/p>\n

See full article here<\/a><\/p>\n

Hindawi Veterinary Medicine International – https:\/\/doi.org\/10.1155\/2022\/5997320
\nReceived 4 January 2022; Accepted 23 February 2022; Published 22 March 2022<\/p>\n","protected":false},"excerpt":{"rendered":"

Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions Manuela Madeddu, Stefano Marelli, Ahmad Abdel Sayed, Fabio Mosca, Silvia Cerolini and Luisa Zaniboni Department of Veterinary Medicine and Animal Sciences, University of Milan, via dell\u2019Universit\u00e0 6, Lodi 26900, Italy Abstract: In order to preserve endangered […]<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"faq_category":[148,244],"class_list":["post-34671","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-animal-es","faq_category-bird-es"],"yoast_head":"\nAssessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions - HT CASA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/assessment-of-sperm-viability-and-computer-assisted-motility-analysis-in-budgerigars-melopsittacus-undulatus-effect-of-several-in-vitro-processing-conditions\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions - 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