{"id":35391,"date":"2022-07-29T17:18:35","date_gmt":"2022-07-29T15:18:35","guid":{"rendered":"https:\/\/www.micropticsl.com\/faq-items\/influence-of-sperm-cryopreservation-on-sperm-motility-and-proakap4-concentration-in-mice\/"},"modified":"2022-07-29T17:18:35","modified_gmt":"2022-07-29T15:18:35","slug":"influence-of-sperm-cryopreservation-on-sperm-motility-and-proakap4-concentration-in-mice","status":"publish","type":"avada_faq","link":"https:\/\/www.micropticsl.com\/es\/faq-items\/influence-of-sperm-cryopreservation-on-sperm-motility-and-proakap4-concentration-in-mice\/","title":{"rendered":"Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice"},"content":{"rendered":"<h3>Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice<\/h3>\n<p>Auke Boersma, Jasmin Primus, Bettina Wagner, Veronika Broukal, Lill Andersen, Barbara Pachner, Maik Dahlhoff, Thomas R\u00fclicke, Kerstin E. Auer<\/p>\n<p><em>Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria; Department of Radiology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Berlin, Germany<br \/>\n<\/em><\/p>\n<p><strong>Abstract:<\/strong><\/p>\n<p><strong>Background:<\/strong> The protein proAKAP4 is crucial for sperm motility and has been suggested as an indicator of male fertility. We determined the relationship between proAKAP4 concentration and sperm motility parameters in mice, and investigated the effects of cryopreservation on these variables.<\/p>\n<p><strong>Methods:<\/strong> Computer-assisted sperm analysis and ELISA were applied to determine sperm motility and proAKAP4 concentration in fresh and frozen-thawed epididymal sperm of SWISS, B6D2F1, C57BL\/6N, and BALB\/c mice.<\/p>\n<p><strong>Results:<\/strong> ProAKAP4 levels ranged between 12 and 89\u2009ng\/ml and did not differ between fresh and frozen-thawed samples, or between strains. We found a negative relationship between proAKAP4 levels and some sperm motility parameters. Sperm traits differed between strains, and cryopreservation negatively affected sperm velocity but not sperm direction parameters.<\/p>\n<p><strong>Conclusion:<\/strong> ProAKAP4 levels in epididymal mouse spermatozoa were unaffected by cryopreservation, highlighting the robustness of this parameter as a potentially time-independent marker for sperm motility and fertility. The high individual variation in proAKAP4 levels supports the potential role of proAKAP4 as a marker for sperm quality, though we found no positive, and even negative relationships between proAKAP4 levels and some sperm motility parameters. Future studies have to investigate the significance of proAKAP4 as an indicator for fertility in mice.<\/p>\n<p>See full article <a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/rmb2.12480\" target=\"_blank\" rel=\"noopener noreferrer\">here<\/a><\/p>\n<p>Reprod Med Biol. 2022;21:e12480 &#8211; https:\/\/doi.org\/10.1002\/rmb2.12480<br \/>\nReceived: 20 April 2022 \/ Accepted: 4 July 2022 \/ First published: 29 July 2022<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice Auke Boersma, Jasmin Primus, Bettina Wagner, Veronika Broukal, Lill Andersen, Barbara Pachner, Maik Dahlhoff, Thomas R\u00fclicke, Kerstin E. Auer Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria; Department of Radiology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Berlin, Germany <a href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/influence-of-sperm-cryopreservation-on-sperm-motility-and-proakap4-concentration-in-mice\/\"> [&#8230;]<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"faq_category":[148,161],"class_list":["post-35391","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-animal-es","faq_category-rodent-es"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice - HT CASA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/influence-of-sperm-cryopreservation-on-sperm-motility-and-proakap4-concentration-in-mice\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice - HT CASA\" \/>\n<meta property=\"og:description\" content=\"Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice Auke Boersma, Jasmin Primus, Bettina Wagner, Veronika Broukal, Lill Andersen, Barbara Pachner, Maik Dahlhoff, Thomas R\u00fclicke, Kerstin E. 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