{"id":8185,"date":"2004-08-01T00:00:09","date_gmt":"2004-07-31T23:00:09","guid":{"rendered":"https:\/\/www.micropticsl.com\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-2\/"},"modified":"2016-12-30T09:48:37","modified_gmt":"2016-12-30T08:48:37","slug":"use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3","status":"publish","type":"avada_faq","link":"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/","title":{"rendered":"Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen"},"content":{"rendered":"<h3>Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen<\/h3>\n<p>Martinez-Pastor F, Johannisson A, Gil J, Kaabi M, Anel L, Paz P, Rodriguez-Martinez H.<\/p>\n<p><em>Department of Anatomy and Histology, Swedish University of Agricultural Sciences (SLU), Box 7039, Uppsala SE-750-07, Sweden<\/em><\/p>\n<p>Cryopreservation of semen imposes deleterious effects on spermatozoa, either killing a certain proportion of cells or causing subtle damages on sperm function in the surviving population, changes not easily revealed by conventional assays. We have tested three functional assessment techniques in frozen-thawed ram semen from six adult rams, cryopreserved following eight different protocols (four extenders, and glycerol being added at two temperatures). Semen samples were thawed and the following analyses were carried out: motility (CASA), membrane integrity (Hoescht 33258 and fluorometry), chromatin status (chromatin stability test and fluorescence-assisted cell sorting, FACS) and mitochondrial activity (JC-1 and FACS). Fluorometry outcome did not correlate with the other parameters and showed large variation, albeit discriminating among cryopreservation techniques ( <div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-overflow:visible;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last fusion-column-no-min-height\" style=\"--awb-bg-size:cover;--awb-margin-bottom:0px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\">[Formula: see text] ). Mitochondrial activity correlated, but with low values, with total and progressive motility. However, good sperm motility and high velocity values were associated to high mitochondrial membrane potential. The chromatin stability assay was also successfully carried out, and had a good relationship with male factor (%COMP [Formula: see text] and SD [Formula: see text] parameters). In conclusion, fluorometric assessment of membrane integrity albeit rendering poor results, merits improvement, being a low-cost and handy technique, especially for work in the field. On the other hand, both assessments of chromatin stability and mitochondrial status (JC-1 staining), combined with FACS, are reliable techniques that can be used for the functional assessment of frozen-thawed ram semen.<\/p>\n<p>Anim Reprod Sci. 2004 Aug;84(1-2):121-33.<i> <\/i><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen Martinez-Pastor F, Johannisson A, Gil J, Kaabi M, Anel L, Paz P, Rodriguez-Martinez H. Department of Anatomy and Histology, Swedish University of Agricultural Sciences (SLU), Box 7039, Uppsala SE-750-07, Sweden Cryopreservation of semen imposes deleterious effects <a href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/\"> [&#8230;]<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"faq_category":[148,156],"class_list":["post-8185","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-animal-es","faq_category-ram-es"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen - HT CASA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen - HT CASA\" \/>\n<meta property=\"og:description\" content=\"Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen Martinez-Pastor F, Johannisson A, Gil J, Kaabi M, Anel L, Paz P, Rodriguez-Martinez H. Department of Anatomy and Histology, Swedish University of Agricultural Sciences (SLU), Box 7039, Uppsala SE-750-07, Sweden Cryopreservation of semen imposes deleterious effects [...]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/\" \/>\n<meta property=\"og:site_name\" content=\"HT CASA\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/HamiltonThorneInc\" \/>\n<meta property=\"article:modified_time\" content=\"2016-12-30T08:48:37+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@HamiltonThorne\" \/>\n<meta name=\"twitter:label1\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"TechArticle\",\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/\"},\"author\":{\"name\":\"microptic\",\"@id\":\"https:\/\/www.micropticsl.com\/#\/schema\/person\/f44383b138fe27e5c0ed406429b01139\"},\"headline\":\"Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen\",\"datePublished\":\"2004-07-31T23:00:09+00:00\",\"dateModified\":\"2016-12-30T08:48:37+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/\"},\"wordCount\":372,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/www.micropticsl.com\/#organization\"},\"inLanguage\":\"es\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/\",\"url\":\"https:\/\/www.micropticsl.com\/es\/faq-items\/use-of-chromatin-stability-assay-mitochondrial-stain-jc-1-and-fluorometric-assessment-of-plasma-membrane-to-evaluate-frozen-thawed-ram-semen-3\/\",\"name\":\"Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen - 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