{"id":12933,"date":"2015-07-07T11:30:54","date_gmt":"2015-07-07T10:30:54","guid":{"rendered":"https:\/\/www.micropticsl.com\/rattox-casa-2\/"},"modified":"2017-09-05T09:58:51","modified_gmt":"2017-09-05T07:58:51","slug":"rattox-casa-2","status":"publish","type":"post","link":"https:\/\/www.micropticsl.com\/es\/rattox-casa-2\/","title":{"rendered":"RatTox CASA"},"content":{"rendered":"<h2>RatTox CASA: A whole new revolution in toxicology testing<\/h2>\n<p>There is a great need to use the least invasive systems to test the effects of potential effects of chemicals, pesticides, radiation and many others on biological systems. Many researchers resorted to cell and tissue cultures as model systems. While these systems have many advantages, they have the disadvantage of not being able to measure the interactions of different organ systems in the organism\u2019s body in relation to potential toxic substances. Furthermore, even if an animal is exposed experimentally and under serious ethical scrutiny to potentially toxic substances or substances they may enhance body functions, which model system will\/should be used to measure\/evaluate this?<\/p>\n<p>Microptic S.L. in conjunction with consultants and researchers has developed a novel system called RatTox making use of rat\/mouse sperm to evaluate the effect of chemicals\/radiation.\u00a0 The RatTox \u201cpackage\u201d consists of four devoted modules, sperm motility\/concentration, morphology\/morphometry, fragmentation and vitality to automatically and accordingly objectively evaluate normal versus treated sperm.<\/p>\n<p>One of the great challenges of this project is\/was to establish what are normal sperm characteristics at this level of CASA quantification? There are no clear reports available on what the cut-off points for sperm motility (kinematics), vitality, fragmentation but particularly for normal morphology may be in a rat\/mouse model. Fortunately there are a number of users in South Africa (three Universities), France and Greece who started to exchange data and in conjunction with Microptic S.L. to establish at least preliminary cut-off points. We are in the process to publish some of the morphology\/morphometry data soon as a multi-international project (See Fig below).<\/p>\n<p>So what do researchers do in the mean time in the \u201cso called absence\u201d of rat\/mouse reference sperm values like we have for humans and domestic animals? The solution is fortunately still possible using SCA CASA objectively. But how? Researchers do not need to use so called reference values but just need to express the treatment values for e.g. motility and morphology as a percentage of the controls. This is a valid approach but surely the ideal would be to also have the cut-off values \u2013 more realistic also having the absolute values.<\/p>\n<p>Furthermore, the good news is that there appears to be more interest globally to use the RatTox system for future toxicological studies. This is not surprising in view of the fact that the protocols are available for all four SCA CASA modules and in practice they work extremely well for Rat\/Mouse sperm.\u00a0 Accordingly the CASA RatTox modules present a new and innovative approach to test the effect of a multitude of substances (positive or negative) on a biological system and has great potential.<\/p>\n<p><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-overflow:visible;--awb-margin-top:40px;--awb-background-color:rgba(255,255,255,0);--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last fusion-column-no-min-height\" style=\"--awb-bg-size:cover;--awb-margin-bottom:0px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p style=\"text-align: center;\"><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-2 hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-overflow:visible;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last fusion-column-no-min-height\" style=\"--awb-bg-size:cover;--awb-margin-bottom:0px;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-image-element fusion-image-align-center in-legacy-container\" style=\"text-align:center;--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><div class=\"imageframe-align-center\"><span class=\" fusion-imageframe imageframe-dropshadow imageframe-1 hover-type-none\" style=\"-webkit-box-shadow: 3px 3px 7px rgba(0,0,0,0.3);box-shadow: 3px 3px 7px rgba(0,0,0,0.3);\"><img decoding=\"async\" width=\"480\" height=\"398\" alt=\"rat_tox_casa_1\" title=\"rat_tox_casa_1\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27480%27%20height%3D%27398%27%20viewBox%3D%270%200%20480%20398%27%3E%3Crect%20width%3D%27480%27%20height%3D%27398%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/www.micropticsl.com\/wp-content\/uploads\/2015\/07\/rat_tox_casa_1.png\" class=\"lazyload img-responsive wp-image-20163\"\/><\/span><\/div><\/div>\n<p style=\"text-align: center;\"><em>Rat sperm stained with SpermBlue automatically analyzed for sperm head and midpiece characteristics.<\/em><\/p>\n<p style=\"text-align: left;\"><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:40px;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p style=\"text-align: center;\"><div class=\"fusion-image-element fusion-image-align-center in-legacy-container\" style=\"text-align:center;--awb-caption-title-font-family:var(--h2_typography-font-family);--awb-caption-title-font-weight:var(--h2_typography-font-weight);--awb-caption-title-font-style:var(--h2_typography-font-style);--awb-caption-title-size:var(--h2_typography-font-size);--awb-caption-title-transform:var(--h2_typography-text-transform);--awb-caption-title-line-height:var(--h2_typography-line-height);--awb-caption-title-letter-spacing:var(--h2_typography-letter-spacing);\"><div class=\"imageframe-align-center\"><span class=\" fusion-imageframe imageframe-dropshadow imageframe-2 hover-type-none\" style=\"-webkit-box-shadow: 3px 3px 7px rgba(0,0,0,0.3);box-shadow: 3px 3px 7px rgba(0,0,0,0.3);\"><img decoding=\"async\" width=\"480\" height=\"337\" alt=\"rat_tox_casa_2\" title=\"rat_tox_casa_2\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27480%27%20height%3D%27337%27%20viewBox%3D%270%200%20480%20337%27%3E%3Crect%20width%3D%27480%27%20height%3D%27337%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/www.micropticsl.com\/wp-content\/uploads\/2015\/07\/rat_tox_casa_2.png\" class=\"lazyload img-responsive wp-image-20165\"\/><\/span><\/div><\/div>\n<p style=\"text-align: center;\"><em>Table showing typical control morphometric values for sperm analyzed above.<br \/>\nCut-off values have been determined for each morphometric parameter.<\/em><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Gerhard van der Horst (PhD, PhD)<br \/>\n<\/strong>Senior Consultant<strong><br \/>\n<\/strong>MICROPTIC S.L.<\/p>\n<div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:40px;width:100%;\"><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p><a title=\"Sistema CASA SCA evolution Sperm Class Analyzer\" href=\"https:\/\/www.micropticsl.com\/es\/?page_id=7616\"><img class=\"lazyload\" decoding=\"async\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27665%27%20height%3D%27100%27%20viewBox%3D%270%200%20665%20100%27%3E%3Crect%20width%3D%27665%27%20height%3D%27100%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/www.micropticsl.com\/wp-content\/uploads\/2013\/09\/banner_sca_evolution_casa_system_esp.png\" alt=\"banner_sca_evolution_casa_system_esp\" width=\"665\" height=\"100\" \/><\/a><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"<p>RatTox CASA: A whole new revolution in toxicology testing There is a great need to use the least invasive systems to test the effects of potential effects of chemicals, pesticides, radiation and many others on biological systems. Many researchers resorted to cell and tissue cultures as model systems. While these systems have many advantages, they <a href=\"https:\/\/www.micropticsl.com\/es\/rattox-casa-2\/\"> [&#8230;]<\/a><\/p>\n","protected":false},"author":1,"featured_media":6021,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[143],"tags":[368,360,262,734],"class_list":["post-12933","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog-es","tag-microptic-es","tag-sca-es","tag-toxicology","tag-toxicology-es"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>RatTox CASA - HT CASA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.micropticsl.com\/rattox-casa\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"RatTox CASA - HT CASA\" \/>\n<meta property=\"og:description\" content=\"RatTox CASA: A whole new revolution in toxicology testing There is a great need to use the least invasive systems to test the effects of potential effects of chemicals, pesticides, radiation and many others on biological systems. 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