{"id":8791,"date":"2014-05-13T08:25:30","date_gmt":"2014-05-13T07:25:30","guid":{"rendered":"https:\/\/www.micropticsl.com\/sperm-cryopreservation-animal-diluents-and-automatic-analysis-2\/"},"modified":"2017-09-05T09:56:42","modified_gmt":"2017-09-05T07:56:42","slug":"sperm-cryopreservation-animal-diluents-and-automatic-analysis-3","status":"publish","type":"post","link":"https:\/\/www.micropticsl.com\/es\/sperm-cryopreservation-animal-diluents-and-automatic-analysis-3\/","title":{"rendered":"Sperm Cryopreservation: Animal diluents and automatic analysis"},"content":{"rendered":"

The first observations concerning low-temperature preservation of spermatozoa<\/b> date from 1776, when the Italian physiologist Spallanzani noted that spermatozoa cooled in snow became inactive but could be revived on warming, although, successful cryopreservation<\/b> protocols date only from the 1950s.<\/p>\n

In 1949, the single most important development came with the discovery by Polge, Smith and Parkes that Glycerol could act as a cryoprotectant from spermatozoa.<\/p>\n

History of Semen Cryopreservation <\/b><\/p>\n

1776 – First observations on the effects of freezing temperatures on human sperm
\n1866 – First banks for frozen human semen suggested
\n1938 – Sperm survives at temperatures as low as -269\u00b0C, and stored at -79\u00b0
\n1949 – Glycerol first used as protective agent for bovine spermatozoa<\/p>\n

suntrust loans<\/a><\/div>\n

1953 – \u00abDry Ice\u00bb preservation method developed
\n1953 – First successful human pregnancy using frozen sperm
\n1957 – Tyler Medical Clinic\/Westwood Cryobank offers freezing of semen
\n1960 – Sperm frozen and stored using liquid nitrogen<\/p>\n

Source:\u00a0 Sherman, \u00abHistorical Synopsis of Human Cryobanking,\u00bb 1979<\/p>\n

Since then, the cryopreservation and artificial insemination have become very frequent in the Assisted Reproduction, both in human and animal.<\/p>\n

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\"frozen_semen_straws\"<\/span><\/div><\/div>\n

Frozen semen straws<\/em><\/p>\n

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One of the critical points, especially in the animal field where artificial insemination became the standard, is the use of diluents containing egg yolk or milk, widely used as cryopreservant, despite other not containing any animal products have demonstrated to be cleaner.<\/p>\n

Besides, it is easy to find publications expressing that this semen diluents containing egg yolk may pose hygienic risks and are difficult to standardize.<\/p>\n

Nevertheless, if the egg yolk diluents is the preferred choice, CASA SCA\u00ae has the option to make the analysis of motility and concentration under fluorescence to avoid any interference with these extender particles.<\/p>\n

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\"bull_sperm_sample_containing_egg_yolk\"<\/span><\/div><\/div>\n

Bull sperm sample containing egg yolk. On the left: brightfield analysis. On the right: Fluorescence analysis with FluoCount<\/em><\/p>\n

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Samples must be stained with FluoCount (Hoechst based fluorochrome) and incubated during 5 minutes to be able to become visible under fluorescence and then can be automatically analyzed.<\/p>\n

 <\/p>\n

Bibliography:<\/p>\n

Cryopreservation of Mammalian Semen, Mark R. Curry<\/em><\/p>\n

In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen.\u00a0 V. A. Aires, K. Hinsch, F. Mueller-Schloesser, K. Bogner, S. Mueller-Schloesser, E. Hinsch<\/em><\/p>\n

Comparative quality assessment of buffalo (Bubalus bubalis) semen chilled (5\u00b0C) in egg yolk- and soya milk-based extenders. A K Singh, V K Singh, B M Narwade, T K Mohanty, S K Atreja<\/em><\/p>\n

 <\/p>\n

Ms Mercedes Dad\u00edn
\n<\/strong>Biologist- Marketing Manager
\nMICROPTIC S.L.<\/p>\n

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\"banner_sca_evolution_casa_system_esp\"<\/a>

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The first observations concerning low-temperature preservation of spermatozoa date from 1776, when the Italian physiologist Spallanzani noted that spermatozoa cooled in snow became inactive but could be revived on warming, although, successful cryopreservation protocols date only from the 1950s. 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