{"id":8491,"date":"2014-04-15T09:55:07","date_gmt":"2014-04-15T08:55:07","guid":{"rendered":"https:\/\/www.micropticsl.com\/?page_id=8491"},"modified":"2025-04-30T12:13:52","modified_gmt":"2025-04-30T10:13:52","slug":"goldcyto-dna","status":"publish","type":"page","link":"https:\/\/www.micropticsl.com\/fr\/produits\/consommables\/fragmentation-adn\/goldcyto-dna\/","title":{"rendered":"GoldCyto DNA"},"content":{"rendered":"
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Kit pour le test de Fragmentation d’ADN<\/h2><\/span>
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\"Kit<\/a><\/span><\/div><\/div>\n
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Cliquez sur l\u2019image pour l\u2019agrandir<\/em><\/p>\n

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Le kit GoldCyto DNA<\/strong> est un test simple permettant d’analyser la fragmentation de l’ADN<\/strong> des spermatozo\u00efdes d’\u00e9chantillons d’humain et d’animal.<\/p>\n

La m\u00e9thode est bas\u00e9e sur l’analyse de la dispersion de la chromatine dans le spermatozo\u00efde<\/strong> (SDC) (Fern\u00e1ndez et al., J. Androl 24:59\u201366, 2003; Fertil Steril 84:833-842, 2005).<\/p>\n

Les spermatzo\u00efdes sans fixer se m\u00e9langent avec un microgel d’agarose inerte\u00a0 dans une lame pr\u00e9-trait\u00e9e. un traitement initial avec de l’acide d\u00e9naturalise l’ADN des spermatozo\u00efdes dont l’ADN reste intact. Ensuite,\u00a0 l’\u00e9chantillon est trait\u00e9 avec une solution de lysis laquelle casse la membrane cellulaire en \u00e9liminant la plupart\u00a0 des prot\u00e9ines du noyau. En l’absence de dommages dans l’ADN, se produisent des grands halos autour de la t\u00eate du spermatozo\u00efde. Par contre, les spermatozo\u00efdes ayant l’ADN fragment\u00e9 restent insensibles \u00e0 la d\u00e9naturalisation et par cons\u00e9quent, il n’y a pas de dispersion de la chromatine (petits halos ou absents).<\/p>\n

Compatible avec \"\"<\/p>\n

FOR RESEARCH USE ONLY<\/em><\/p>\n

Plus d’informations :<\/p>\n

Demande d\u2019informations<\/span><\/a><\/div>\n
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Contenu du kit GoldCyto :<\/h3>\n<\/i>\nLames pr\u00e9-trait\u00e9es
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<\/div>\n<\/i>\nTubes Eppendorf avec de l’agarose \u00e0 point de fusion bas
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<\/div>\n<\/i>\nBouteille de tampon de phosphate salin
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<\/div>\n<\/i>\nBouteille d’agent d\u00e9naturant
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<\/div>\n<\/i>\nBouteille de solution lysis
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<\/div>\n<\/i>\nBouteille de solution de colorant T-A
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<\/div>\n<\/i>\nBouteille de colorant T-B<\/p>\n
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Informations suppl\u00e9mentaires :<\/h3>\n
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Kit pour 20 analyses<\/td>\n<\/tr>\n
R\u00e9f\u00e9rence : GC-SCD-20<\/td>\n<\/tr>\n
Catalogue: T\u00e9l\u00e9charger le catalogue<\/a> \"pdf_thumbnail\"<\/td>\n<\/tr>\n
Protocole:
\nT\u00e9l\u00e9charger le protocole<\/a> \"pdf_thumbnail\"
\nT\u00e9l\u00e9charger le protocole pour animal<\/a> \"pdf_thumbnail\"<\/td>\n<\/tr>\n
Voir tutoriel GoldCyto DNA<\/a> \"YouTube\"<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":28426,"parent":8489,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-8491","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nGoldCyto DNA - HT CASA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.micropticsl.com\/fr\/produits\/consommables\/fragmentation-adn\/goldcyto-dna\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"GoldCyto DNA - HT CASA\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.micropticsl.com\/fr\/produits\/consommables\/fragmentation-adn\/goldcyto-dna\/\" \/>\n<meta property=\"og:site_name\" content=\"HT CASA\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/HamiltonThorneInc\" \/>\n<meta property=\"article:modified_time\" content=\"2025-04-30T10:13:52+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.micropticsl.com\/wp-content\/uploads\/2017\/04\/featured_disposables.png\" \/>\n\t<meta property=\"og:image:width\" content=\"300\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@HamiltonThorne\" \/>\n<meta name=\"twitter:label1\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.micropticsl.com\/fr\/produits\/consommables\/fragmentation-adn\/goldcyto-dna\/\",\"url\":\"https:\/\/www.micropticsl.com\/fr\/produits\/consommables\/fragmentation-adn\/goldcyto-dna\/\",\"name\":\"GoldCyto DNA - 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