SCA® Leukocytes

SCA® Leukocytes is a module for the automatic analysis of human peroxidase-positive leukocytes, in samples treated with the Leukocytes slides SCA.

A semen sample is mixed with a specific substrate for the peroxidase enzyme. If there is peroxidase, the substrate containing hydrogen peroxide will be reduced. At the same time, diaminobenzidine (DAB) will be oxidized and turn into an insoluble brown product.

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SCA® Leukocytes main features

Leukocytes analysis with Leukocytes slides SCA - SCA® Leukocytes

Analysis is performed in brightfield, in samples treated with the Leukocytes slides SCA.

Leukocytes automatic analysis - SCA® Leukocytes

Cells are detected automatically by the presence of the enzyme peroxidase in cells.

Additional information

Human
Edition
Research
Edition
Creating new analysis configurationsicon_correcticon_correct
Modification of the optics, microscope scales and image capture optionsicon_correcticon_correct
Modification of the particle area cut off parameter for cell detectionicon_correcticon_correct
Motorised stageicon_correcticon_correct
Species available: Humanicon_correcticon_correct
Human
Edition
Research
Edition
Specific digital Basler camera with 780×580icon_correcticon_correct
Specific digital Basler camera with 1280×1024icon_correcticon_correct
Human
Edition
Research
Edition
Total cells countedicon_correcticon_correct
Percentage of the counted cells with peroxidase-positive leukocytesicon_correcticon_correct
Concentration (M/ml) and Total number (M/ejaculate) with peroxidase-positive leukocytesicon_correcticon_correct
Captured imagesicon_correcticon_correct
Human
Edition
Research
Edition
SCA® dataShareicon_correcticon_correct
SCA® Stage Controllericon_correcticon_correct
SCA® Captureicon_correcticon_correct
SCA® Viewericon_correcticon_correct
SCA® Editoricon_correcticon_correct
The assessment is in brightfield microscopy at 40x.
Peroxidase-positive leukocytes analysis is evaluated using Leukocytes slides SCA.
Possibility to add or eliminate cells and/or captures fields.
Calculation of basic parametres: Percentage and concentration of peroxidase-positive leukocytes.
Visualization of the analysis mask superimposed to the original image.
Image capture takes 1 second per field.
Allows capture up to 30 fields of the sample.
Counting of peroxidase-positive leukocytes.
Number, percentage and concentration of peroxidase-positive leukocytes.

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