SCA® Morphology

SCA® Morphology is a module that permits the automatic morphometry analysis and morphology classification of human stained sperm samples observed in brightfield microscopy.

This analysis module is also
available for Veterinary and Toxicology.

There are enormous variation in the sperm morphology, which make its assessment quite complex, but several studies have recovered spermatozoa from female reproductive tract, specially in postcoital endoncervical mucus, and from the surface of zona pellucida and have helped to define the appearance of potentially fertilizing, that is, morphologically normal, spermazoa in some species. (Medkveld et al,. 1991: Liu & Baker, 1992a)

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SCA® Morphology last news

Vacuoles and tails analysis - SCA® Morphology

Vacuoles and tails

Automatic analysis of vacuoles and tails with SCA® Morphology.

Ready for Morpholog slides SCA - SCA® Morphology

Morphology slides SCA

Ready for the new Morphology slides SCA pre-stained slides.

Multiple defects at the same time - SCA® Morphology

Multiple defects

Assessment of all possible defects at the same time: TZI, MAI and SDI.

Analysis and detection improved - SCA® Morphology

Analysis improved

Morphology analysis and sperm detection improved.

SCA® Morphology main features

Automatic detection and analysis - SCA® Morphology

SCA® Morphology detects and analyses the spermatozoa automatically.

Complete analysis - SCA® Morphology

Simultaneously evaluates the dimensions of the acrosome, head, midpiece, tail and the presence of vacuoles.

Criteria selection - SCA® Morphology

Spermatozoa are recorded as normal or abnormal, following established criteria: W.H.O., Strict Kruger-Tygerberg, David modified, Adaptative or Customized.

Full automatic analysis - SCA® Morphology

Optionally, analysis with motorized stage and SCA® Stage controller is available, improving the capture velocity and making all the process automatic.

Multiple stains - SCA® Morphology

Several staining are compatible: Sperm Stain, SpermBlue, Diff-Quik, Papanicoulau, Shorr or Morphology slides SCA and SCA® contains the appropriate correction factors. *

Statistics - SCA® Morphology

SCA® Morphology is an effective and user-friendly tool for research, allowing the implementation of new terminology and estimations in sperm morphology.

* Morphometric dimensions of the human sperm head depend on the staining method used
L. Maree, S.S. du Plessis, R. Menkveld and G. van der Horst

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Additional information

Creating new analysis configurationsicon_correcticon_correct
Modification of analysis box sizeicon_correcticon_correct
B/W or color capture, Scale, image capture optionsicon_correcticon_correct
Motorized stageicon_correcticon_correct
Human classification: W.H.O. or David Modified Classification
Human classification: W.H.O, Kruger-tygerberg, David modified and User defined icon_correcticon_correct
All staining options: Diff-Quik, Sperm Stain, SpermBlue®, Hemacolor, Papanicolau, Shorr, Cell-Vu Prestained Slides.icon_correcticon_correct
Particle area modificationicon_correcticon_correct
Modification of the Morphology classification / Morphometric valuesicon_correcticon_correct
Species available: Humanicon_correcticon_correct
Species available: All (human, and all animals, including rat and mouse)icon_correct
Species available: All animals with oval sperm head (boar, bull, stallion, etc)icon_correct
Species available: rat and mouseicon_correct
Specific digital Basler camera with 780×580 resolutionicon_correcticon_correct
Specific digital Basler camera with 1280×1024 resolutionicon_correcticon_correct
Total and percentage normal/abnormal sperm analysedicon_correcticon_correct
Basic classification: normal and head, midpiece and tail defects, cytoplasmic dropletsicon_correcticon_correct
TZI, MIA, SDI, ERC Indexicon_correcticon_correct
Total analyzed and percentage of the spermatozoa with the next morphologic values (*): normal size, acrosome and midpiece; micro, macro, normal shape, tapered, thin, round, pyriform, amorphous, abnormal midpiece in size, angle, insertion, normal and abnormal tail, short, irregular, rolled, multiple, without, angulation.
* Morphologic values might change depending on the classification selected.
Morphometric values: head length, width,area, perimeter, ellipticity, elongation, surface roughness, regularityicon_correcticon_correct
Captured images and distribution chartsicon_correcticon_correct
SCA® dataShareicon_correcticon_correct
SCA® Stage Controller
SCA® Captureicon_correcticon_correct
SCA® Viewericon_correcticon_correct
SCA® Editoricon_correcticon_correct
Analysis under brightfield microscopy, transmitting light to view a specimen that is stained.
SCA Morphology analyzes the head, acrosome and midpiece form, shape and size.
Tails can be analyzed automatically with the use of the specific high resolution digital camera 1280×1024 or manually if desired.
Automatic vacuoles analysis is available using the Sperm Blue® staining kit.
There are several Compatible staining methods depending on the specimen: Diff quik, Papanicoulau, Sperm Blue®, Sperm Stain, Shorr and CellVu prestained slides for human. Sperm  Blue® for all species, which allows a complete analysis, including acrosome visualization. For each of the staining used the software has corrected factors.
Allows the creation of User defined matrix for the classification into normal or abnormal sperm
Additional descriptions of the Morphologic values can be created
Modifiable parameters are: Size of the capture box, staining methods and Classification criteria
SCA® permits to capture and analyse up to 500 spermatozoa per sample
Head length, width, area, perimeter, ellipticity, elongation, roughness, regularity
Acrosome, percentage of head size
Vacuoles, presence and percentage of head size (with SpermBlue® staining)
 Midpiece width, area, insertion angle
Tail length
Calculation of the following basic parameters: Total analyzed and Percentage of normal and abnormal spermatozoa
Total analyzed and Percentage of normal spermatozoa; with head, midpiece and tail defects; and cytoplasmic droplets.
Total and percentage of counted spermatozoa having Just one defect, in the Head, Midpiece or Tail.
Total and percentage of counted spermatozoa having Multiple defects combined as: Head+ Midpiece, Head+Tail, Midpiece+Tail, Head+Midpiece+Tail
Morphologic values*: Total and percentage of analysed spermatozoa having Normal size, Micro, Macro, Normal shape, Tapered, Thin, Round, Pyriform, Amorphous; Normal  and Abnormal Acrosome; Normal midpiece, Abnormal midpiece size, angle and insertion; Normal tail, abnormal, short, Irregular, Rolled, Multiple, Without, Angulation
Average and deviation of these morphometric values: Head lengh, width, area, perimeter, ellipticity, elongation, roughness, regularity; Midpiece width, area and angle; Acrosome
Teratozoospermy index (TZI), Deformity index(SDI), Multiple anomalies index (MIA), Number of spermatozoa with Excess residual cytoplasm (ERC)

* this might change according the chosen Morphology classification

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