SCA® Vitality

SCA® Vitality is a module that automatically provides the percentage of live and dead spermatozoa in a human semen sample.

This analysis module is also
available for Veterinary and Toxicology.

Sperm Vitality is estimated by assessing the membrane integrity of the cells, and is fundamental for samples having less than 40% progressive motile spermatozoa. The percentage of the viable cells normally exceeds that of motile cells.

Vitality assessment can be performed with Vitality slides SCA (in brightfield) or with FluoVit kit (under fluorescence).

More information:

SCA® Vitality last news

Brightfield analysis - SCA® Vitality

Brightfield analysis

New vitality analysis in brightfield, in samples stained with Vitality slides SCA.

Ready for Vitality slides SCA - SCA® Vitality

Vitality slides SCA

Ready for the new Vitality slides SCA to assess the Vitality in brightfield.

Interactive threshold modification - SCA® Vitality

Interactive threshold

Interactive threshold modification for sperm classification.

Analysis and detection improved - SCA® Vitality

Analysis improved

Vitality analysis and sperm detection improved.

SCA® Vitality main features

Vitality analysis with Vitality slides SCA or FluoVit - SCA® Vitality

Analysis is performed in brightfield, in samples stained with Vitality slides SCA and can be also performed under fluorescence microscopy with FluoVit kit.

Vitality automatic analysis with Vitality slides SCA - SCA® Vitality

Spermatozoa are recognized and classified automatically (in brightfield, alive sperm in white and dead sperm in red).

Vitality automatic analysis with FluoVit - SCA® Vitality

Fluorescence analysis allows the differentiation between alive sperm (in blue), and the dead sperm (in red).

Additional information

Creating new analysis configurationsicon_correcticon_correct
Modification of the optics, microscope scales and image capture optionsicon_correcticon_correct
Modification of the particle area cut off parameter for cell detectionicon_correcticon_correct
Motorised stageicon_correcticon_correct
Species available: Humanicon_correcticon_correct
Species available: All (human, and all animals, including rat and mouse)icon_correct
Species available: All animals with oval sperm headicon_correct
Species available: rat and mouseicon_correct
Specific digital Basler camera with 780×580icon_correcticon_correct
Specific digital Basler camera with 1280×1024icon_correcticon_correct
Total sperm countedicon_correcticon_correct
Percentage of the counted sperm that are alive and deadicon_correcticon_correct
Captured images and distribution chartsicon_correcticon_correct
SCA® dataShareicon_correcticon_correct
SCA® Stage Controllericon_correcticon_correct
SCA® Captureicon_correcticon_correct
SCA® Viewericon_correcticon_correct
SCA® Editoricon_correcticon_correct
The assessment is under fluorescence microscopy at 20x:  The specimen is illuminated with light of a specific wavelength, which is absorbed by the fluorochromes, causing them to emit light of longer wavelengths, i.e., of a different color than the absorbed light .
Vitality of spermatozoa is evaluated indirectly though the integrity of the cellular membrane using vital fluorescence staining FluoVit®.
Vitality analysis can be also assessed in brightfield at 20x using the Vitality slides SCA.
Possibility to add or eliminate spermatozoa and/or captures fields
Calculation of basic parametres: Number and percentage of live and dead spermatozoa.
Visualization of the analysis mask superimposed to the original image.
Image capture takes 1 second per field
Allows capture up to 30 fields of the sample
Counting of live and dead spermatozoa by color detection
Number and percentage of live and dead spermatozoa

Image gallery